The Pursuit of God is Tozer's most famous and widely read book. This book in particular has reached all corners of the world. It is considered a Christian classic and is still read and listened to by many faithful Christians to this day. It was written and published while he was a pastor in Chicago.

In The Pursuit of God, Tozer writes about why people pursue a relationship with God and how one can best go about doing so. Much of Tozer's doctrine can be found in this book. Tozer writes, “We need never shout across the spaces to an absent God. He is nearer than our own soul, closer than our most secret thoughts.” In Chapter 1, Tozer explains that the reason one desires to pursue God is because God gave one that desire. God gives one the impulse, but one must “follow hard after him”. Tozer implores readers to strip to the “bare essentials” and present themselves before God without any efforts to impress him.