Little Women was an immediate commercial and critical success, with readers eager for more about the characters. Alcott quickly completed a second volume (titled Good Wives in the United Kingdom, though the name originated with the publisher and not Alcott). It was also met with success. The two volumes were issued in 1880 as a single novel titled Little Women. Alcott subsequently wrote two sequels to her popular work, both also featuring the March sisters: Little Men (1871) and Jo's Boys (1886).

The novel has been said to address three major themes: "domesticity, work, and true love, all of them interdependent and each necessary to the achievement of its heroine's individual identity." According to Sarah Elbert, Alcott created a new form of literature, one that took elements from romantic children's fiction and combined it with others from sentimental novels, resulting in a totally new genre. Elbert argues that within Little Women can be found the first vision of the "All-American girl" and that her various aspects are embodied in the differing March sisters.

The book has been translated into numerous languages, and frequently adapted for stage and screen.